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Data managers

What are Data Managers?

Data Managers are a special class of Galaxy tool which allows for the download and/or creation of data that is stored within Tool Data Tables and their underlying flat (e.g. .loc) files. These tools handle e.g. the creation of indexes and the addition of entries/lines to the data table / .loc file via the Galaxy admin interface.

Data Managers can be defined locally or installed through the Tool Shed.

A Video Introduction

For a video overview on Data Managers, see this presentation from GCC2013.

Tutorial

The most up-to-date methods, including how to use Data Manager repositories in the Tool Shed: GCC2014 TrainingDay

What Kind of Data is Supported

The Data Manager framework supports any kind of built-in (“pre-cached”) data that a tool developer would like to make available via a Tool Data Table. This includes reference genomes, indexes on a reference genome, BLAST databases, protein or pathway domain databases, and so-on. This built-in data does not need to be associated with any type of reference, build, or dbkey (genomic or otherwise), but, in many cases, Tool Data Table entries and their Data Manager will be tied to a specific genomic build.

Graphical Overview of Interplay between Built-in Data and Galaxy Tools

../_images/data_managers_schematic_overview.png

Galaxy Data Manager XML File

The XML File for a Galaxy Data Manager, generally referred to as the “data manager config file”, serves a number of purposes. It defines the availability of Data Managers to a Galaxy instance. It does this by specifying the id of the Data Manager and the Data Manager tool that is associated with it. It also contains a listing of the Tool Data Tables that can be added to by the Data Manager. It also specifies how to manipulate the raw column values provided by the Data Manager Tool and under what directory structure to place the finalized data values.

Pay attention to the following when creating a new Data Manager:

  1. Make sure your XML is valid - Improper XML will most likely cause Galaxy to not load your Data Managers. The easiest way to validate your XML is just to open the XML file itself in e.g. Firefox, which will either parse the file and display it, or show the error and its location in large letters.
  2. Don’t forget to restart Galaxy - Galaxy loads and parses XML at run-time, which means you’ll have to restart it after updating any XML files. The same does not apply if you only update an executable.
  3. Make sure you use an id that is unique within your Galaxy instance - Galaxy can only load one Data Manager having an the same ID at a single time.
  4. When completed, make your Data Manager available in a ToolShed and install it from there - This will avoid any possible collisions due to non-unique IDs, as specialized name-spacing is utilized when Data Managers are installed from a ToolShed.

A Galaxy Data Manager’s config file consists of a subset of the following XML tag sets - each of these is described in detail in the following sections.

Details of XML tag sets

<data_managers> tag set

The outer-most tag set. It contains no attributes. Any number of <data_manager> tags can be included within it.

<data_manager> tag set

This tag defines a particular Data Manager. Any number of <data_table> tags can be included within it.

attribute values required example details
tool_file A string* yes tool_file="data_manager/twobit_builder.xml" This is the filename of the Data Manager Tool’s XML file, relative to the Galaxy Root. Multiple Data Managers can use the same Tool, but doing so would require “id” to be declared.
id A string* no id="twobit_builder" Must be unique across all Data Managers; should be lowercase and contain only letters, numbers, and underscores. While technically optional, it is a best-practice to specify this value. When not specified, it will use the id of the underlying Data Manager Tool.

The following is an example that contains all of the attributes described above.

<data_manager tool_file="data_manager/twobit_builder.xml" id="twobit_builder">

<data_table> tag set

This tag defines a Tool Data Table to add entries to. Any number of <data_table> tags can be used. Each <data_table> tag will contain an <output> tagset.

attribute values required example details
name A string* yes name="twobit" This is the name of the Tool Data Table.

The following is an example that contains all of the attributes described above.

<data_table name="twobit">

<output> tag set

This tag defines how to handle the output of the Data Manager Tool. It has no attributes, but contains one or more <column> tag sets.

The following is an example that contains all of the attributes described above.

<output>

<column> tag set

This tag defines a particular Tool Data Table column that will be set. Any number of <column> tags can be used. Each <column> tag may contain <move> and / or <value_translation> tagsets, which are optional.

attribute values required example details
name A string* yes name="value" This is the name of Tool Data Table column.
output_ref A string* no output_ref="out_file" Name of the Data Manager Tool’s output file to use for additional processing within e.g. a tag.

The following is an example that contains all of the attributes described above.

<column name="path" output_ref="out_file" >

<move> tag set

This tag defines how to handle moving files from within the Data Manager Tool output’s extra_files_path into the final storage location used for the Tool Data Table entry. Individual files or the entire directory contents can be moved. Move tag sets contain a <source> and a <target> tag set.

attribute values required example details
type A string* no <move type="file"> This can be either ‘file’ or ‘directory’. Default is ‘directory’.
relativize_symlinks True or False no relativize_symlinks="True" Whether or not to relativize created existing symlinks in moved target. Default is False.

The following is an example that contains all of the attributes described above.

<move type="file" relativize_symlinks="False">

<source> tag set

This tag defines the source location within a <move> tag set. When not specified, it defaults to the entire extra_files_path of the output reference dataset. Both the base attribute and the text of the <source> tag are treated as Cheetah templates, with the columns names specified in the <column> tagsets available as variables (with values taken from the corresponding data table entries. The strings produced for the base attribute and the tag text should resolve to a single line.

attribute values required example details
base A string Template no <source base=""> The base/root path to use for the source. When not provided, it defaults to the extra_files_path of the output dataset.
TEXT A string Template no <source>${path}</source> This defines the value of the source, relative to the base

The following is an example that contains the most common usage, where the value provided by the Data Manager Tool, relative to the extra_files_path, is used as the source.

<source>${path}</source>

<target> tag set

This tag defines the target location within a <move> tag set. When not specified, it defaults to the galaxy_data_manager_data_path configuration value. The values of the base and the tag text are treated as templates as with the <source> tag. In addition the variables from the <column> tagset the value of galaxy_data_manager_data_path configuration value is available using the ${GALAXY_DATA_MANAGER_DATA_PATH} variable.

attribute values required example details
base A string Template no <source base="${GALAXY_DATA_MANAGER_DATA_PATH}"> The base/root path to use for the target. When not specified, it defaults to the galaxy_data_manager_data_path configuration value.
TEXT A string Template no <target base="${GALAXY_DATA_MANAGER_DATA_PATH}">${dbkey}/seq/${path}</target> This defines the value of the target (destination), relative to the base

The following is an example that contains a common usage, where a target value is constructed using several of the values provided by the Data Manager Tool, relative to the galaxy_data_manager_data_path, is used as the source.

<target base="${GALAXY_DATA_MANAGER_DATA_PATH}">${dbkey}/seq/${path}</target>

<value_translation> tag set

This tag allows using templating to modify the value provided by the Data Manager Tool into the actual value that should be stored within the Tool Data Table. There can be any number of value translations provided for an output. The value translations are performed in the order presented in the XML. It is important to note that a move will occur before the value translations are performed.

attribute values required example details
type A string no type="template" The type of value translation to perform. Currently “template” and “function” are supported.

The following is an example that contains a common usage, where a value is constructed using several of the values provided by the Data Manager Tool and that value is then turned into an absolute path. If <value_translation> is a string (not a function) it is treated as a template, much like <source> and <target>, and must return a single line string.

<value_translation>${GALAXY_DATA_MANAGER_DATA_PATH}/${value}/seq/${path}</value_translation>
<value_translation type="function">abspath</value_translation>

Bringing it all Together, an example

Assume that we have a Data Manager Tool that provides the following named values:

name value
value sacCer2
path sacCer2.2bit

and creates an output named “out_file”, with an extra_files_path containing a file ‘sacCer2.2bit’. (The primary dataset file contains JSON that provides the above values)

and has a Data Manager configuration defined as:

<data_managers>
    <data_manager tool_file="data_manager/twobit_builder.xml" id="twobit_builder">
        <data_table name="twobit">
            <output>
                <column name="value" />
                <column name="path" output_ref="out_file" >
                    <move type="file">
                        <source>${path}</source>
                        <target base="${GALAXY_DATA_MANAGER_DATA_PATH}">${value}/seq/${path}</target>
                    </move>
                    <value_translation>${GALAXY_DATA_MANAGER_DATA_PATH}/${value}/seq/${path}</value_translation>
                    <value_translation type="function">abspath</value_translation>
                </column>
            </output>
        </data_table>
    </data_manager>
<data_managers>

The result is:

name value
value sacCer2
path ${ABSOLUTE_PATH_OF_CONFIGURED_GALAXY_DATA_MANAGER_DATA_PATH}/sacCer2/seq/sacCer2.2bit

and the “sacCer2.2bit” file has been moved into the location specified by path.

Data Manager JSON Syntax

Data Manager Tools are required to use JSON to communicate the new Tool Data Table values back to the Data Manager. JSON can also optionally be used to provide the input parameter values to the Data Manager Tool, but this is not required.

Returning Values to the Data Manager

A Data Manager Tool must provide the new values for the Tool Data Table Entries via a JSON dictionary.

  1. A single dictionary, with the key data_tables is required to be present within the root JSON dictionary.
  2. The data_tables dictionary is keyed by the name of the Tool Data Table receiving new entries. Any number of named tables can be specified.
  3. The value for the named Tool Data Table is a list of dictionaries or has add and remove as keys each with a list of dictionaries.
  4. Each of these dictionaries contains the values that will be provided to the Data Manager and modified as per the configuration defined within the Data Manager XML Syntax.

Example 1 JSON Output from Data Manager Tool to Galaxy

{
  "data_tables":{
    "all_fasta":[
      {
        "path":"sacCer2.fa",
        "dbkey":"sacCer2",
        "name":"S. cerevisiae June 2008 (SGD/sacCer2) (sacCer2)",
        "value":"sacCer2"
      }
    ]
  }
}

This creates a new entry in the Tool Data Table:

Example 2 JSON Output from Data Manager Tool to Galaxy

{
  "data_tables":{
    "all_fasta": {
      "add": [
        {
          "path":"sacCer2.fa",
          "dbkey":"sacCer2",
          "name":"S. cerevisiae June 2008 (SGD/sacCer2) (sacCer2)",
          "value":"sacCer2"
        }
      ],
      "remove": [
      ],
    }
  }
}

Returning Values to the Data Manager

Taking the input values of a Data Manager Tool and converting it into a usable set of command-line arguments and options can be quite complicated in many cases, especially when considering that the underlying Data Manager Tool Executable will likely take those options and convert them into a set of valued objects within the executable/script itself before performing its operations.

To simplify this process, Data Manager Tools will automatically have their parameter values JSONified and provided as the content of the output dataset. This will allow the executable / script to simply read and parse the JSON data and have a complete collection of the Tool and Job parameters to use within the tool. Using this methodology is not required, however, and a Data Manager Tool developer is free to explicitly declare any number of the Tool parameters explicitly to the command-line.

Example JSON input to tool

{
  "param_dict":{
    "__datatypes_config__":"/Users/dan/galaxy-central/database/tmp/tmphyQRH3",
    "__get_data_table_entry__":"<function get_data_table_entry at 0x10d435b90>",
    "userId":"1",
    "userEmail":"dan@bx.psu.edu",
    "dbkey":"sacCer2",
    "sequence_desc":"",
    "GALAXY_DATA_INDEX_DIR":"/Users/dan/galaxy-central/tool-data",
    "__admin_users__":"dan@bx.psu.edu",
    "__app__":"galaxy.app:UniverseApplication",
    "__user_email__":"dan@bx.psu.edu",
    "sequence_name":"",
    "GALAXY_DATATYPES_CONF_FILE":"/Users/dan/galaxy-central/database/tmp/tmphyQRH3",
    "__user_name__":"danb",
    "sequence_id":"",
    "reference_source":{
      "reference_source_selector":"ncbi",
      "requested_identifier":"sacCer2",
      "__current_case__":"1"
    },
    "__new_file_path__":"/Users/dan/galaxy-central/database/tmp",
    "__user_id__":"1",
    "out_file":"/Users/dan/galaxy-central/database/files/000/dataset_200.dat",
    "GALAXY_ROOT_DIR":"/Users/dan/galaxy-central",
    "__tool_data_path__":"/Users/dan/galaxy-central/tool-data",
    "__root_dir__":"/Users/dan/galaxy-central",
    "chromInfo":"/Users/dan/galaxy-central/tool-data/shared/ucsc/chrom/sacCer2.len"
  },
  "output_data":[
    {
      "extra_files_path":"/Users/dan/galaxy-central/database/job_working_directory/000/202/dataset_200_files",
      "file_name":"/Users/dan/galaxy-central/database/files/000/dataset_200.dat",
      "ext":"data_manager_json",
      "out_data_name":"out_file",
      "hda_id":201,
      "dataset_id":200
    }
  ],
  "job_config":{
    "GALAXY_ROOT_DIR":"/Users/dan/galaxy-central",
    "GALAXY_DATATYPES_CONF_FILE":"/Users/dan/galaxy-central/database/tmp/tmphyQRH3",
    "TOOL_PROVIDED_JOB_METADATA_FILE":"galaxy.json"
  }
}

Running Data Manager Tools using the API

See scripts/api/data_manager_example_execute.py for an example script.

Writing Data Manager Tests

Writing a Data Manager test is similar to writing a test for any other Galaxy Tool. For an example, please see at http://testtoolshed.g2.bx.psu.edu/view/blankenberg/data_manager_example_blastdb_ncbi_update_blastdb.

Running Data Manager Tests

Data Managers can be tested using the built-in run_tests.sh script. All installed Data Managers can be tested, or individual Data Managers can be tested.

To test all: sh run_tests.sh -data_managers

To test a single Data Manager byid: sh run_tests.sh -data_managers -id data_manager_id

Testing in the ToolShed

All Data Managers deposited within the ToolShed are tested using the nightly testing framework.

Defining Data Managers

Data Manager Components

Data Managers are composed of two components:

  • Data Manager configuration (e.g. data_manager_conf.xml)
  • Data Manager Tool

Data Manager Configuration

The Data Manager Configuration (e.g. data_manager_conf.xml) defines the set of available Data Managers using an XML description. Each Data Manager can add entries to one or more Tool Data Tables. For each Tool Data Table under consideration, the expected output entry columns, and how to handle the Data Manager Tool results, are defined.

Data Manager Tool

A Data Manager Tool is a special class of Galaxy Tool. Data Manager Tools do not appear in the standard Tool Panel and can only be accessed by a Galaxy Administrator. Additionally, the initial content of a Data Manager’s output file contains a JSON dictionary with a listing of the Tool parameters and Job settings (i.e. they are a type of OutputParameterJSONTool, this is also available for DataSourceTools). There is no requirement for the underlying Data Manager tool to make use of these contents, but they are provided as a handy way to transfer all of the tool and job parameters without requiring a different command-line argument for each necessary piece of information.

The primary difference between a standard Galaxy Tool and a Data Manager Tool is that the primary output dataset of a Data Manager Tool must be a file containing a JSON description of the new entries to add to a Tool Data Table. The on-disk content to be referenced by the Data Manager Tool, if any, is stored within the extra_files_path of the output dataset created by the tool.

A data manager tool can use a conda environment if the target Galaxy is version 18.09 or above (specified in the tool’s XML file).

Data Manager Server Configuration Options

In your galaxy.yml ensure these settings are set:

# Data manager configuration options
enable_data_manager_user_view: true
data_manager_config_file: data_manager_conf.xml
shed_data_manager_config_file: shed_data_manager_conf.xml
galaxy_data_manager_data_path: tool-data

Where enable_data_manager_user_view allows non-admin users to view the available data that has been managed.

Where data_manager_config_file defines the local XML file to use for loading the configurations of locally defined data managers.

Where shed_data_manager_config_file defines the local XML file to use for saving and loading the configurations of locally defined data managers.

Where galaxy_data_manager_data_path defines the location to use for storing the files created by Data Managers. When not configured it defaults to the value of tool_data_path.

An example single entry data_manager_config_file

<?xml version="1.0"?>
<data_managers> <!-- The root element -->
    <data_manager tool_file="data_manager/fetch_genome_all_fasta.xml" id="fetch_genome_all_fasta"> <!-- Defines a single Data Manager Tool that can update one or more Data Tables -->
        <data_table name="all_fasta"> <!-- Defines a Data Table to be modified. -->
            <output> <!-- Handle the output of the Data Manager Tool -->
                <column name="value" /> <!-- columns that are going to be specified by the Data Manager Tool -->
                <column name="dbkey" />
                <column name="name" />
                <column name="path" output_ref="out_file" >  <!-- The value of this column will be modified based upon data in "out_file". example value "phiX.fa" -->
                    <move type="file"> <!-- Moving a file from the extra files path of "out_file" -->
                        <source>${path}</source> <!-- File name within the extra files path -->
                        <target base="${GALAXY_DATA_MANAGER_DATA_PATH}">${dbkey}/seq/${path}</target> <!-- Target Location to store the file, directories are created as needed -->
                    </move>
                    <value_translation>${GALAXY_DATA_MANAGER_DATA_PATH}/${dbkey}/seq/${path}</value_translation> <!-- Store this value in the final Data Table -->
                </column>
            </output>
        </data_table>
    <!-- additional data_tables can be configured from a single Data Manager -->
    </data_manager>
</data_managers>

An example data_manager/fetch_genome_all_fasta.xml

This Tool Config calls a Python script data_manager_fetch_genome_all_fasta.py and provides a single file out_file and the description from the dbkey dropdown menu for input.

The starting contents of out_file contain information from Galaxy about the tool, including input parameter values, in the JSON format. Data Manager tools are expected to be able to parse this file. The Data Manager tool will also put the return output values for its results in this file; additional files to be moved can be placed in the extra_files_path of out_file.

<tool id="data_manager_fetch_genome_all_fasta" name="Reference Genome" version="0.0.1" tool_type="manage_data">
    <description>fetching</description>
    <command interpreter="python">data_manager_fetch_genome_all_fasta.py "${out_file}" --dbkey_description ${ dbkey.get_display_text() }</command>
    <inputs>
        <param name="dbkey" type="genomebuild" label="DBKEY to assign to data" />
        <param type="text" name="sequence_name" value="" label="Name of sequence" />
        <param type="text" name="sequence_desc" value="" label="Description of sequence" />
        <param type="text" name="sequence_id" value="" label="ID for sequence" />
        <conditional name="reference_source">
            <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
                <option value="ucsc">UCSC</option>
                <option value="ncbi">NCBI</option>
                <option value="url">URL</option>
                <option value="history">History</option>
                <option value="directory">Directory on Server</option>
            </param>
            <when value="ucsc">
                <param type="text" name="requested_dbkey" value="" label="UCSC's DBKEY for source FASTA" optional="False" />
            </when>
            <when value="ncbi">
                <param type="text" name="requested_identifier" value="" label="NCBI identifier" optional="False" />
            </when>
            <when value="url">
                <param type="text" area="True" name="user_url" value="http://" label="URLs" optional="False" />
            </when>
            <when value="history">
                <param name="input_fasta" type="data" format="fasta" label="FASTA File" multiple="False" optional="False" />
            </when>
            <when value="directory">
                <param type="text" name="fasta_filename" value="" label="Full path to FASTA File on disk" optional="False" />
                <param type="boolean" name="create_symlink" truevalue="create_symlink" falsevalue="copy_file" label="Create symlink to orignal data instead of copying" checked="False" />
            </when>
        </conditional>
    </inputs>
    <outputs>
        <data name="out_file" format="data_manager_json"/>
    </outputs>
    <!--
    <tests>
        <test>
            DON'T FORGET TO DEFINE SOME TOOL TESTS
        </test>
    </tests>
    -->
    <help>
**What it does**

Fetches a reference genome from various sources (UCSC, NCBI, URL, Galaxy History, or a server directory) and populates the "all_fasta" data table.

------

.. class:: infomark

**Notice:** If you leave name, description, or id blank, it will be generated automatically.

    </help>
</tool>

An example data_manager_fetch_genome_all_fasta.py

#!/usr/bin/env python
#Dan Blankenberg

import sys
import os
import tempfile
import shutil
import optparse
import urllib2
from ftplib import FTP
import tarfile

from galaxy.util.json import from_json_string, to_json_string

CHUNK_SIZE = 2**20 #1mb

def cleanup_before_exit( tmp_dir ):
    if tmp_dir and os.path.exists( tmp_dir ):
        shutil.rmtree( tmp_dir )

def stop_err(msg):
    sys.stderr.write(msg)
    sys.exit(1)

def get_dbkey_id_name( params, dbkey_description=None):
    dbkey = params['param_dict']['dbkey']
    #TODO: ensure sequence_id is unique and does not already appear in location file
    sequence_id = params['param_dict']['sequence_id']
    if not sequence_id:
        sequence_id = dbkey #uuid.uuid4() generate and use an uuid instead?

    sequence_name = params['param_dict']['sequence_name']
    if not sequence_name:
        sequence_name = dbkey_description
        if not sequence_name:
            sequence_name = dbkey
    return dbkey, sequence_id, sequence_name

def download_from_ucsc( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name ):
    UCSC_FTP_SERVER = 'hgdownload.cse.ucsc.edu'
    UCSC_CHROM_FA_FILENAME = 'chromFa.tar.gz' #FIXME: this file is actually variable...
    UCSC_DOWNLOAD_PATH = '/goldenPath/%s/bigZips/' + UCSC_CHROM_FA_FILENAME
    COMPRESSED_EXTENSIONS = [ '.tar.gz', '.tar.bz2', '.zip', '.fa.gz', '.fa.bz2' ]

    email = params['param_dict']['__user_email__']
    if not email:
        email = 'anonymous@example.com'

    ucsc_dbkey = params['param_dict']['reference_source']['requested_dbkey'] or dbkey
    ftp = FTP( UCSC_FTP_SERVER )
    ftp.login( 'anonymous', email )
    ucsc_file_name = UCSC_DOWNLOAD_PATH % ucsc_dbkey

    tmp_dir = tempfile.mkdtemp( prefix='tmp-data-manager-ucsc-' )
    ucsc_fasta_filename = os.path.join( tmp_dir, UCSC_CHROM_FA_FILENAME )

    fasta_base_filename = "%s.fa" % sequence_id
    fasta_filename = os.path.join( target_directory, fasta_base_filename )
    fasta_writer = open( fasta_filename, 'wb+' )

    tmp_extract_dir = os.path.join ( tmp_dir, 'extracted_fasta' )
    os.mkdir( tmp_extract_dir )

    tmp_fasta = open( ucsc_fasta_filename, 'wb+' )

    ftp.retrbinary( 'RETR %s' % ucsc_file_name, tmp_fasta.write )

    tmp_fasta.seek( 0 )
    fasta_tar = tarfile.open( fileobj=tmp_fasta, mode='r:*' )

    fasta_reader = [ fasta_tar.extractfile( member ) for member in fasta_tar.getmembers() ]

    data_table_entry = _stream_fasta_to_file( fasta_reader, target_directory, dbkey, sequence_id, sequence_name )
    _add_data_table_entry( data_manager_dict, data_table_entry )

    fasta_tar.close()
    tmp_fasta.close()
    cleanup_before_exit( tmp_dir )

def download_from_ncbi( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name ):
    NCBI_DOWNLOAD_URL = 'http://togows.dbcls.jp/entry/ncbi-nucleotide/%s.fasta' #FIXME: taken from dave's genome manager...why some japan site?

    requested_identifier = params['param_dict']['reference_source']['requested_identifier']
    url = NCBI_DOWNLOAD_URL % requested_identifier
    fasta_reader = urllib2.urlopen( url )

    data_table_entry = _stream_fasta_to_file( fasta_reader, target_directory, dbkey, sequence_id, sequence_name )
    _add_data_table_entry( data_manager_dict, data_table_entry )

def download_from_url( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name ):
    urls = filter( bool, map( lambda x: x.strip(), params['param_dict']['reference_source']['user_url'].split( '\n' ) ) )
    fasta_reader = [ urllib2.urlopen( url ) for url in urls ]

    data_table_entry = _stream_fasta_to_file( fasta_reader, target_directory, dbkey, sequence_id, sequence_name )
    _add_data_table_entry( data_manager_dict, data_table_entry )

def download_from_history( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name ):
    #TODO: allow multiple FASTA input files
    input_filename = params['param_dict']['reference_source']['input_fasta']
    if isinstance( input_filename, list ):
        fasta_reader = [ open( filename, 'rb' ) for filename in input_filename ]
    else:
        fasta_reader = open( input_filename )

    data_table_entry = _stream_fasta_to_file( fasta_reader, target_directory, dbkey, sequence_id, sequence_name )
    _add_data_table_entry( data_manager_dict, data_table_entry )

def copy_from_directory( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name ):
    input_filename = params['param_dict']['reference_source']['fasta_filename']
    create_symlink = params['param_dict']['reference_source']['create_symlink'] == 'create_symlink'
    if create_symlink:
        data_table_entry = _create_symlink( input_filename, target_directory, dbkey, sequence_id, sequence_name )
    else:
        if isinstance( input_filename, list ):
            fasta_reader = [ open( filename, 'rb' ) for filename in input_filename ]
        else:
            fasta_reader = open( input_filename )
        data_table_entry = _stream_fasta_to_file( fasta_reader, target_directory, dbkey, sequence_id, sequence_name )
    _add_data_table_entry( data_manager_dict, data_table_entry )

def _add_data_table_entry( data_manager_dict, data_table_entry ):
    data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} )
    data_manager_dict['data_tables']['all_fasta'] = data_manager_dict['data_tables'].get( 'all_fasta', [] )
    data_manager_dict['data_tables']['all_fasta'].append( data_table_entry )
    return data_manager_dict

def _stream_fasta_to_file( fasta_stream, target_directory, dbkey, sequence_id, sequence_name, close_stream=True ):
    fasta_base_filename = "%s.fa" % sequence_id
    fasta_filename = os.path.join( target_directory, fasta_base_filename )
    fasta_writer = open( fasta_filename, 'wb+' )

    if isinstance( fasta_stream, list ) and len( fasta_stream ) == 1:
        fasta_stream = fasta_stream[0]

    if isinstance( fasta_stream, list ):
        last_char = None
        for fh in fasta_stream:
            if last_char not in [ None, '\n', '\r' ]:
                fasta_writer.write( '\n' )
            while True:
                data = fh.read( CHUNK_SIZE )
                if data:
                    fasta_writer.write( data )
                    last_char = data[-1]
                else:
                    break
            if close_stream:
                fh.close()
    else:
        while True:
            data = fasta_stream.read( CHUNK_SIZE )
            if data:
                fasta_writer.write( data )
            else:
                break
        if close_stream:
            fasta_stream.close()

    fasta_writer.close()

    return dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_filename )

def _create_symlink( input_filename, target_directory, dbkey, sequence_id, sequence_name ):
    fasta_base_filename = "%s.fa" % sequence_id
    fasta_filename = os.path.join( target_directory, fasta_base_filename )
    os.symlink( input_filename, fasta_filename )
    return dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_filename )

REFERENCE_SOURCE_TO_DOWNLOAD = dict( ucsc=download_from_ucsc, ncbi=download_from_ncbi, url=download_from_url, history=download_from_history, directory=copy_from_directory )

def main():
    #Parse Command Line
    parser = optparse.OptionParser()
    parser.add_option( '-d', '--dbkey_description', dest='dbkey_description', action='store', type="string", default=None, help='dbkey_description' )
    (options, args) = parser.parse_args()

    filename = args[0]

    params = from_json_string( open( filename ).read() )
    target_directory = params[ 'output_data' ][0]['extra_files_path']
    os.mkdir( target_directory )
    data_manager_dict = {}

    dbkey, sequence_id, sequence_name = get_dbkey_id_name( params, dbkey_description=options.dbkey_description )

    if dbkey in [ None, *, '?' ]:
        raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) )

    #Fetch the FASTA
    REFERENCE_SOURCE_TO_DOWNLOAD[ params['param_dict']['reference_source']['reference_source_selector'] ]( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name )

    #save info to json file
    open( filename, 'wb' ).write( to_json_string( data_manager_dict ) )

if __name__ == "__main__": main()

Example JSON Output from tool to galaxy, dbkey is sacCer2

{
  "data_tables":{
    "all_fasta":[
      {
        "path":"sacCer2.fa",
        "dbkey":"sacCer2",
        "name":"S. cerevisiae June 2008 (SGD/sacCer2) (sacCer2)",
        "value":"sacCer2"
      }
    ]
  }
}

New Entry in Data Table, dbkey is sacCer2